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| 1. |  | CAMOZZI, M.G.M; SATURNINO, K. C.; MACHADO, M.R.F.; GASTAL, G.D.A.; MOREIRA, C. N.; ALVES, B. G. Cystic endometrial hyperplasia-pyometra syndrome impairs the preantral follicle reserve in domestic bitches (Canis familiaris). Reproductive Biology, December 2023, Volume 23, Issue 4, 100813. https://doi.org/10.1016/j.repbio.2023.100813 Article history: Received 19 February 2023; Received in revised form 25 September 2023; Accepted 27 September 2023; Available online 11 October 2023. -- Corresponding author: Alves, B.G.; Postgraduate Program in Animal Bioscience, Federal...| Biblioteca(s): INIA Las Brujas. |
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
28/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
A - 1 |
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Autor : |
ORSOMANDO, G.; LORENZI, M.; RAFFAELLI, N.; DALLA RIZZA, M.; MEZZETTI, B.; RUGGIERI, S. |
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Afiliación : |
GIUSEPPE ORSOMANDO, Dipto. Biotecnol. Agrarie Ambientali, Facoltà di Agraria, University of Ancona, Italy; MARIA LORENZI, Dipto. Biotecnol. Agrarie Ambientali, Facoltà di Agraria, University of Ancona, Italy; NADIA RAFFAELLI, Istituto di Biochimica, Facolta di Medicina e Chirurgia, University of Ancona, Italy; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Dipto. Biotecnol. Agrarie Ambientali, Facoltà di Agraria, University of Ancona, Italy; BRUNO MEZZETTI, Dipto. Biotecnol. Agrarie Ambientali, Facoltà di Agraria, University of Ancona, Italy; SILVERIO RUGGIERI, Dipto. Biotecnol. Agrarie Ambientali, Facoltà di Agraria, University of Ancona, Italy; Dipto. Biotecnol. Agrarie Ambientali, Università di Ancona, Italy. |
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Título : |
Phytotoxic protein PcF, purification, characterization, and cDNA sequencing of a novel hydroxyproline-containing factor secreted by the strawberry pathogen phytophthora cactorum. |
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Fecha de publicación : |
2001 |
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Fuente / Imprenta : |
Journal of Biological Chemistry, 15 June 2001, Volume 276, Issue 24, pp. 21578-21584. |
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ISSN : |
0021-9258 (print) |
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DOI : |
10.1074/jbc.M101377200 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received for publication, February 13, 2001 / In revised form, March 21, 2001 / Published, JBC Papers in Press, March 21, 2001.
This work was supported by a grant from the Regione Marche 5b.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked
"advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
This work is dedicated to the memory of Professor Pasquale Rosati.
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AF354650. |
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Contenido : |
ABSTRACT.
A novel protein factor, named PcF, has been isolated from the culture filtrate of Phytophthora cactorum strain P381 using a highly sensitive leaf necrosis bioassay with tomato seedlings. Isolated PcF protein alone induced leaf necrosis on its host strawberry plant. The primary structure and cDNA sequence of this novel phytotoxic protein was determined, and BLAST searches of Swiss-Prot, EMBL, and GenBank?/EBI data banks showed that PcF shared no significant homology with other known sequences. The 52-residue PcF protein, which contains a 4-hydroxyproline residue along with three S-S bridges, exhibits a high content of acidic sidechains, accounting for its isoelectric point of 4.4. The molecular mass of isolated PcF is 5,622 ± 0.5 Da as determined by mass spectrometry and matches that calculated from the deduced amino acid sequence with cDNA sequencing. The cDNA sequence indicates that PcF is first produced as a larger precursor, comprising an additional N-terminal, 21-residue secretory signal peptide. Maturation of this protein involves the hydroxylation of proline 49, a feature that is unique among other known secreted fungal phytopathogenic proteins. |
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Palabras claves : |
BIODIVERSITY; Fragaria x ananassa; Molecular Sequence Data. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.jbc.org/content/276/24/21578.full.pdf
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Marc : |
LEADER 02675naa a2200253 a 4500
001 1012821
005 2019-10-28
008 2001 bl uuuu u00u1 u #d
022 $a0021-9258 (print)
024 7 $a10.1074/jbc.M101377200$2DOI
100 1 $aORSOMANDO, G.
245 $aPhytotoxic protein PcF, purification, characterization, and cDNA sequencing of a novel hydroxyproline-containing factor secreted by the strawberry pathogen phytophthora cactorum.$h[electronic resource]
260 $c2001
500 $aArticle history: Received for publication, February 13, 2001 / In revised form, March 21, 2001 / Published, JBC Papers in Press, March 21, 2001. This work was supported by a grant from the Regione Marche 5b. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. This work is dedicated to the memory of Professor Pasquale Rosati. The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s) AF354650.
520 $aABSTRACT. A novel protein factor, named PcF, has been isolated from the culture filtrate of Phytophthora cactorum strain P381 using a highly sensitive leaf necrosis bioassay with tomato seedlings. Isolated PcF protein alone induced leaf necrosis on its host strawberry plant. The primary structure and cDNA sequence of this novel phytotoxic protein was determined, and BLAST searches of Swiss-Prot, EMBL, and GenBank?/EBI data banks showed that PcF shared no significant homology with other known sequences. The 52-residue PcF protein, which contains a 4-hydroxyproline residue along with three S-S bridges, exhibits a high content of acidic sidechains, accounting for its isoelectric point of 4.4. The molecular mass of isolated PcF is 5,622 ± 0.5 Da as determined by mass spectrometry and matches that calculated from the deduced amino acid sequence with cDNA sequencing. The cDNA sequence indicates that PcF is first produced as a larger precursor, comprising an additional N-terminal, 21-residue secretory signal peptide. Maturation of this protein involves the hydroxylation of proline 49, a feature that is unique among other known secreted fungal phytopathogenic proteins.
653 $aBIODIVERSITY
653 $aFragaria x ananassa
653 $aMolecular Sequence Data
700 1 $aLORENZI, M.
700 1 $aRAFFAELLI, N.
700 1 $aDALLA RIZZA, M.
700 1 $aMEZZETTI, B.
700 1 $aRUGGIERI, S.
773 $tJournal of Biological Chemistry, 15 June 2001, Volume 276, Issue 24, pp. 21578-21584.
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